The Horror Of The 1920 S - 1218 Words

One of the bestselling books out of the 1920’s is All Quite on the Western Front. It gives us a look at how brutal and horrifying the war was in that period of time. Describing two stories, the book the precisely chronicles the thoughts of a soldier in World War I (WWI) while simultaneously describing the horrors of all wars. It does not try to make any of its chapters seem unrealistic to the audience for example, each account is not only a separate experience, but a new representation of the fighting. Like all other books, it tells the depth and how it took a great toll on how the world perceived the war. Before, everyone had this romantic theme almost on how the war had such great love stories and its semi-horrifying experiences that had brought them back together. This book has the horrifying tragedies on how a soldier’s life really was in the 20’s. How it took the soldiers emotional and psychical being to a whole new level. The war is seen through the eyes o f Paul Baumer (the main character). His mindset is more superior than his comrades’, but to take on a godly role that he takes to serve as the connections between WWI and all past and future disorders. He represents all man and through him the reader comes to see such human struggle. The story begins before enlistment; the soldiers are stuffed with beef, beans, and happiness. The mens’ futures were certain to be good as they feel satisfied and at peace; each man had shelter and a mess tin full for the evening. AfterShow MoreRelatedAnalysis Of The Movie 1920 American Film 1318 Words   |  6 Pages1920’s American Film During the 1920s, American Film was at the peak of its glory. 1920s Film was the biggest form of entertainment and a weekly pastime for millions of Americans, regardless of race and social background. Silent films continued to improve and innovate the film industry. Hollywood established themselves as an American force and produced hundreds of silent films. Also, Hollywood became the birthplace of â€Å"movie stars† such as Janet Gaynor, Rudolph Valentino, and Charlie Chaplin. MovieRead MoreThe Evolution of Horror Films Essay1595 Words   |  7 Pagesterror are all just a few things to expect when watching a modern day horror film. What is horror? Horror can be defined as an intense feeling of fear, shock, or disgust. (Wilson) The description of horror is not very pleasant, but for some reason horror films are extremely popular. Why is this so? People are addicted to the synthetic feeling of being terrified. Modern day horror films are very different from the first horror films which date back to the late nineteenth century, but the goal ofRead MoreWhen Karl Freund’S The Mummy (1932) Was Released, The Horror1313 Words   |  6 Pages When Karl Freund’s The Mummy (1932) was released, the horror genre was already ingrained by Universal Studios and other American studios. The 1930s are considered the beginning of the horror genre’s classic phase. Horror films â€Å"primarily engages us affectively and viscerally-its aim seems to be to scare and disgust us, to raise the hair on the back of our necks or make us cover our eyes†. The horror film of the studios years gives physical shape and specific presence to metaphysical, notionsRead MoreImpact Of Sports On Society1371 Words   |  6 Pages1920’s Impact of Sports on Society The 1920’s were an important time period in America’s history. The horrors of World War one were at long last over and society was collectively ready to move on to greener pastures. Americans proceeded vigorously to make life feel more refined than before the war. From the mass production of automobiles and the automation of everything imaginable to the rebirth of African-American culture and fine arts, the world had drastically changed. One such instrument inRead MoreRoaring Twenties : A Decade Of Extravagance939 Words   |  4 PagesTwenties: A Decade of Extravagance The Roaring Twenties was a time of fun and delight the stock market was booming and America was thriving while other countries were experiencing turmoil in aftermath of the First World War. Returning from the horrors of war, writers were inspired by romance and the riches America had. Using their unique creative expression, many of the writings from the Twenties were a commentary on social life, the good and bad. Each story holds a different theme, different messageRead MoreAfrican American Women and Feminists of the 1920s1031 Words   |  4 PagesFeminist of the 1920’s In the 1920’s, blues was a very popular and dominating genre in the music industry. Generally, the blues was sung by African American women because according to the book entitled, â€Å"Blues Legacies and Black Feminism† by Angela Davis, â€Å"†¦The most widely heard individual purveyors of the blues—were women.† (Davis 4) The blues delivers certain emotions such as sadness, loneliness, love, sex, and feelings about the certain circumstances the artist may be going through at the timeRead MoreHitchcocks Film Psycho Essay1575 Words   |  7 PagesHitchcocks Film Psycho Ever since the first horror movies were produced they have attracted huge audiences seeking to be scared, chilled and thrilled. Horror movies are so popular because the audience can get the adrenaline rush of being scared without actually putting themselves in danger, and also the audience ultimately get a rush of relief at the end of the film when the killer is killed. This is the same reason why people go onRead MoreEssay on Ray Bradbury1243 Words   |  5 PagesBradbury was born on August 22, 1920, in a small town of Waukegan, Illinois. His parents were Leonard Spaulding and Esther Moberg Bradbury. His mother, Esther Moberg loved films, she gave her son the middle name Douglas because of Douglas Fairbanks, and she passed her love of films to her son. quot;My mother took me to see everything.....quot; Bradbury explains, quot;Im a child of motion pictures.quot; Prophetically, the first film he saw, at the age of three, was the horror classic quot;The HunchbackRead MoreThe Harlem Renaissance And Marcus Garvey s Universal Negro Improvement Association ( Unia )854 Words   |  4 PagesThe 1920’s were a time of change for African Americans. They were beginning to retain a sense of pride in their background and culture, were becoming more independent socially and economically, and were becoming more militant. Part of this was because of the Great Migration, in which a proliferation of African Americans moved from the Southern states to the Northern states, and the excessive levels of racism and prejudice they faced during the process. African Americans were really starting to makeRead MoreThe Yellow Wallpaper : A Feminist Cry843 Words   |  4 Pagessocietal oppression of women in the patriarchal society of the American nineteenth century. This story is not merely a gothic horror as many critics have stated; although, the story does hold aspects like a broken mind and imprisonment, the story is not com pletely a gothic horror. Paula A. Treichler also interpreted this story as as a feminist cry rather than a gothic horror when she stated the story is â€Å"a fictional challenge to the patriarchal diagnosis of women’s condition, it is also a public critique

Software Engineering Design Rules For All Components

In software engineering, requirements from user met with available components, then following activities needs to be performed 1) Component Qualification: System requirements and architecture define the components that will be required. Reusable components (whether COTS or in house) are normally identified by the characteristics of their interfaces. That is, â€Å"the services that are provided, and the means by which consumers access these services† are described as part of the component interface. But the interface does not provide a complete picture of the degree to which the component will fit the architecture and requirements. The software engineer must use a process of discovery and analysis to qualify each component’s fit. 2) Component Adaptation: we noted that software architecture represents design patterns that are composed of components (units of functionality), connections, and coordination. In essence the architecture defines the design rules for all components, identifying modes of connection and coordination. In some cases, existing reusable components may be mismatched to the ar chitecture’s design rules. These components must be adapted to meet the needs of the architecture or discarded and replaced by other, more suitable components. 3) Component Composition: Architectural style again plays a key role in the way in which software components are integrated to form a working system. By identifying connection and coordination mechanisms (e.g., run-time propertiesShow MoreRelatedThe Elements Of A Collection Framework And Increase The Capability Of Existing Adapted Data Type1424 Words   |  6 PagesAbstract: In software development, there have a complex problem is found out that object, class or similar types of data cannot be plugged in when inference miss matches. Collection framework is used to overcome this problem. A unified interface to store, retrieve and manipulate the elements of a collection is provided by collection framework. A collection framework package contains a set of interface, implementation classes and algorithm such as sorting and searching algorithm. But user cannotRead MoreReuse Of Asset Architecture For Some System1279 Words   |  6 Pagesinvestment. Reused- based software engineering is a software engineering approach where the development process is geared to reusing recent software. One of the best efficient approaches to reuse is to create software product line engineering or application families. In recent years, software reusability has emerged as promising reusability approach, which brings out some important benefits such as increasing reusability of its core assets, while decreasing time to market. Software reusability has focalRead MoreComputer Engineering : Computer Science Department Essay1432 Words   |  6 Pages2016 CSCI665 Software Engineering Midterm Name: Nelson Christian Id:1053366 1) Software Engineering can be defined as set of rules or patterns to follow while working on different aspects of Software. These patterns may vary by person to person, but core concepts remain same. Software involves stages like collecting requirement, designing, development, testing and maintenance and Software engineering is applied to all these stages to create high quality software. 2) Computer Engineering is more likeRead MoreThe Uses and Development of a System1659 Words   |  7 Pagesfrom a different view A set of elements, methods which is explained in detail, procedures a specific path to carry out specific activity’s, to perform a duty or may solve a problem. People from different engineering disciplines have different ideas of what a system is. For example, software engineers usually refer to an integrated set of computer programs as a system. Electrical engineers may refer to complex integrated circuits or an integrated setup of electrical units as a system. Read MoreProfessional Writing in Computer Science Essay1177 Words   |  5 PagesComputer Software Engineer develops software systems to be used by their clients, such as a website used to sell the client’s products. Although software engineers spend the majority of their time programming and testing the software, a key component to the success of a software engineer is the ability to write a thorough Software Requirement Specification (SRS). A SRS documents the requirements and dependencies needed for the software, prior to beginning any programming. Therefore, a Software EngineerRead MoreEssay On Uml Diagrams1139 Words   |  5 Pages UML to Executable Code Generators and source code to UML Generators Sai Priya Anumula, California State University, Fullerton Abstract Code Automation from UML diagrams gains much interest lately in software design, because it has many benefits as it reduces the effort to generate code and moreover automated code is less error prone than writing code manually. However, major challenges in this area include checking consistency of UML models, and ensuring accuracy, maintainability, and efficiencyRead MoreNotes. Water Fall Model:. Introduction:. In 1985, The United1196 Words   |  5 Pageswith software development agents, which stated that the agent shall apply a software development cycle that includes the 6 phases: Requirement Engineering, Analysis Design, Implementation, Integration and Testing, Deployment, Maintenance. Steps of Water Fall Model: REQUIREMENT ENGINEERING: DEFINITION: Requirement engineering is the process of determining user’s need for a new modified product. These features, called requirements, must be quantifiable, relevant and detailed. In software engineeringRead MoreSoftware1632 Words   |  7 PagesPrinciple of Software Engineering Table of Contents Abstract 3 Introduction 4 Boehms First Law 4 Boehms Second law 5 Conways law 5 Parnas Law 6 Corbatà ³ Law 7 Observation 8 Theory 9 Law 9 Question 3 10 Law 11 References 12 Abstract The purpose of the study is to show the capability to understand the set of laws that are the part of principles of the software engineering. In this paper, it is discussed that there are manyRead MoreEnterprise Resource Planning ( Erp )1420 Words   |  6 Pages(304463099) 1. ERP Overview Enterprise Resource Planning (ERP) software items are business application, bundled software. They are utilized to run vast to medium-sized business ventures. The ERP software suppliers assert that their product can oblige each business movement of each class of business undertaking. As a rule, an endeavor depends on various databases to keep up its operations: to encourage the relocation, the ERP software underpins numerous databases for communication and include howeverRead MoreDecision Making Classification Of Terms Of Art And Science1396 Words   |  6 PagesObjectives which need to be classified and placed in terms of order importance, should be fixed or established firstly. - Secondly, alternative actions that are evaluated against all the objectives should be implemented and developed. - Later, provisional decision is an alternative action that is capable of achieving all the objectives and it needs to be estimated for the consequences that are most likely to take place. - Finally, additional steps and decisive actions should be taken to avid the major

Christians Views On Christianity - 942 Words

Christianity is one of the three largest religious in the world and Christians always believe that Juses is the Son of God. â€Å"In the beginning God created the heavens and the earth. Now the earth was formless and empty, darkness was over the surface of the deep, and the Spirit of God was hovering over the waters.† (Genesis 1-3) Christians always believe that the God created the people, and developed the whole people’s life. Christians’ belief is that they need to believe the word of God and these worlds are the foundation of the Christians. Christian practices are divided by denomination, however, the basic elements include private and corporate prayer, studying and reading of the Scriptures, a Sunday worship service and participation in rites such as baptism and communion. Christian worship services basically include singing, prayer and a sermon. A large number of churches have a special ceremony for ordination. Most Christians practice pray at home and also read the Bible. Many Christian will have baptism, when they was an infant or grew up an adult. They need to participate communion in regular time. The morality in Christian said that they need to focus on the relationship between the people and the God, and Christians need to have love. Christianity also emphasizes the confession, repentance, forgiveness, humility and so on. Therefore, â€Å"God created mankind in his own image, in the image of God he created them, male and female he created them.† (Genesis 1-3) Teachings isShow MoreRelatedThe Quakers As Simple People, Good Christians, By The Religious Views Of Christianity885 Words   |  4 PagesEquiano sees the Quakers as simple people, good Christians, who have supported minorities, and who have been fundamental in what he calls,  "breaking the yoke of slavery.† Not only was a Quaker the one who allowed him to purchase his liberty, but Quakers were the leaders of society in favor of abolition. 18) Do you think Equiano’s mind was â€Å"colonized† by the religious views of Christianity? (719-21) For Equiano, Christianity becomes a cornerstone to his identity as a free man. In his work heRead More Saint Augustine Essay1445 Words   |  6 Pagesorthodox Christian doctrine during the early Christian Church. Augustine was born in Northern Africa in AD 354. His father was a pagan and his mother a Christian. Though his parents were not extremely well to do, they had enough money to allow Augustine to obtain an education in the liberal arts. This education will eventually affect how he sees Christianity, especially concerning the use of neo-Platonic ideas in Christian theology. Augustine converted to orthodox Christianity late in lifeRead More Comparing Islam and Christianity Essay735 Words   |  3 Pagesand Christianity Although they share several basic theological ideas, Christianity and Islam differ on countless key characteristics. The most prominent differences include atonement, the identity of God, heaven, view of Jesus, and the perception of the Christian Bible. It is these not-so-subtle contrasts of thoughts that separate the two most prevalent religions in the world. One of the most important differences in Christianity and Islam is the concept of atonement. In Christianity, Jesus’Read MoreThe World s Largest Religion1291 Words   |  6 Pages Final Exam Essay Christianity is the world’s largest religion (Stark, R. 2012. 494). Currently with around two billion followers worldwide who are focused on Jesus Christ. He lived in the â€Å"Holy Land† known as Israel, two-thousand years ago. Christianity outstandingly has a great reach on the world; Christmas is celebrated as the birth of Jesus Christ which has become a government holiday in the United States. Christianity having such a large number of followers is the only religion in the worldRead MoreEffect Of Change During The Post Independence Period1624 Words   |  7 Pagesserved as a hindrance to the involvement of Christians (Safo-Kantanka 1993, 53). Christianity in Ghana When European missionaries arrived on the Gold Coast (now Ghana), they deemed certain indigenous practices unholy and contradictory to Christian teachings. Christianity also affected the institution of chieftaincy in that Christians resisted cocoa farmers, traders and educated men who wanted to share their power with the traditional rulers because Christians opposed the institution of chieftaincy andRead MoreDifferences Between Judaism and Christianity Essays623 Words   |  3 PagesJudaism and Christianity Out of all the major world religions Judaism and Christianity have the closest relationship. There are many difference and many similarities within these religions mainly because Christianity grew out of Judaism. Many people including Jesus and the twelve disciples were Jews (religionfacts.) The main differences are how each religion views Jesus and God, heaven and hell, and the notion of sin. The biggest difference between Judaism and Christianity is their view on God andRead MoreChristianity And The Roman Era845 Words   |  4 PagesChristianity is something that has stirred up a lot of controversy through the ages. During the Roman era, people like Emperor Trajan and Pliny, a Roman governor, did not appreciate Christianity since Christians did not follow the Roman religion. On the other side, Origen defends Christianity by debunking Celsus’s view of it. While Trajan and Pliny found fault in Christianity, Origen saw the potential religion that would allow humans to become better people. During early Christianity, Roman officialsRead MoreThe Rise Of Christianity By Don Nardo1737 Words   |  7 Pagesthe Nicene Creed unified the beliefs of Christianity? The investigation will attempt to answer this question as best as possible. The focused time period will be from 313 AD to 325 AD. This is because practicing Christianity was made legal in Rome in 313 AD and the Nicene Creed was created and made the official statement of Christian beliefs in 325 AD. Also the investigation will focus mainly on events taken place in Rome. Unity, or lack thereof, in Christianity, events before and after the creationRead MoreDefinition Essay : My Worldview1619 Words   |  7 Pagesframework from which we view reality and make sense of life and the world. Worldview is basic on ideology, philosophy, theology, movement, or religion that provides an overreaching approach to understanding God, the world and man’s relations to God and the world. My worldview I’m a Christianity. According to James N Anderson 2014 What’s your worldview? â€Å"Christianity is the largest religion in the world today, Claiming around one-third of the world population. Christians consider to be not merelyRead More The Next Christendom: The Coming of Global Christianity Essay1217 Words   |  5 Pagesnbsp;nbsp;nbsp;nbsp;nbsp;Christianity is becoming extinct. In order for it to continue on it must modernize its beliefs. The average Christian is white, middle class and is from Europe or America. The religion of Islam is expanding quickly and will soon dominate Christianity. These are all common claims made about Christianity that many people believe, yet Philip Jenkins claims and proves them untrue in his book, The Next Christendom: The Coming of Global Christianity. According to his book, Christianity is moving

Henry Jackson Hunt at Gettysburg - 533 Words

Hunt at Gettysburg Henry Jackson Hunt had a positive impact on Gettysburg. Hunt had a strong military background, and was raised in a family of soldiers. He had a lot of achievements before the civil war. He effected Picketts Charge the most but he took part in the action on the other days of the battle. Even after Gettysburg Hunt was still a big help to defeat the South in the Civil War. Hunt had a good military background. Hunt’s father and brother served in the military. Hunt knew that he had to fulfil his family legacy and become a soldier. Hunt went and attended the United States Military Academy. After, Hunt graduated school, he joined the 2nd US Artillery. Hunt was known for his achievements in battle and his keen knowledge in artillery placement. He fought in the Mexican War under Scott Winfield. During the Mexican War Hunt had a cannon to cannon duel and won. When the South broke off from the United States Hunt wasnt the leader of Potomac yet. But soon after the Civil War b roke out Hunt was promoted to the Leader of the Potomac Army Artillery. Historians agree that the reason why lee did not counter-attack the North at bull run is that Hunt had cannons ready to attack when the South came. When battle broke out at Gettysburg Bufford was defending the ground waiting for reinforcements from the North . Hunt was at Taneytown on the first day of Gettysburg. At around seven o’clock Hunt received an order from Meade, the Leader of theShow MoreRelatedReconstruction : The Burning Years10732 Words   |  43 Pagesinterviewers wrote down the African Americans’ answers in stereotypical dialect, or asked leading questions to make slavery sound like it was good for slaves. One former slave, Martin Jackson, spoke in 1937 about the difficulty of getting African Americans to speak honestly about the Civil War and Reconstruction: MARTIN JACKSON: Lots of old slaves closes the door before they tell the truth about their days of slavery. When the door is open, they tell how kind their masters was and how rosy it all was.Read MoreOne Significant Change That Has Occurred in the World Between 1900 and 2005. Explain the Impact This Change Has Made on Our Lives and Why It Is an Important Change.163893 Words   |  656 PagesHistory 53, no. 3 (1993): 575– 600; Klaus J. Bade, â€Å"German Emigration to the United States and Continental Immigration to Germany in the Late Nineteenth and Early Twentieth Centuries,† Central European History 13, no. 4 (1980): 348–377; James H. Jackson, Migration and Urbanization in the Ruhr Valley, 1821–1914 (Atlantic Highlands, NJ: Humanities Press, 1997). 35. Javier Silvestre, â€Å"Internal Migrations in Spain, 1877–1930,† European Review of Economic History 9, no. 2 (2005): 233–265. 36. James

The Nobel Prize in Chemistry Free Essays

In 2014, the Nobel Prize in Chemistry was awarded to Eric Betzig and William Moerner who, working separately, laid the foundation for SMLM. In essence, this method relies on the possibility to turn the fluorescence of individual molecules on and off. Scientists image the same area multiple times, allowing only a few interspersed molecules to glow each time. We will write a custom essay sample on The Nobel Prize in Chemistry or any similar topic only for you Order Now By superimposing these images, a dense super-image can be resolved at the nanolevel. With the development of this technique, Betzig and Moerner were able to overcome Abbe’s diffraction limit, allowing for the production of high resolution images that, before SMLM, had not been possible. Towards the end of the nineteenth century, Ernst Abbe and Lord Rayleigh formulated what is commonly known as the â€Å"diffraction limit† for microscopy. Roughly speaking, this limit states that it is impossible to resolve two elements of a structure that are closer to each other than about half the wavelength (?) in the lateral (x, y) plane and even further apart in the longitudinal (z) plane. Another consequence of the same diffraction limit is that it is not possible to focus a laser beam to a spot of smaller dimension than about ?/2. In the case of light (optical) microscopy, an important tool for the imaging of biological structures, this means that two objects within a distance between 400/2 = 200 nm (far blue) and 700/2 = 350 nm (far red) cannot be resolved. Although this is no real limitation for electron microscopy, in which the wavelength is orders of magnitude smaller, this method is very difficult to use on living cells. For instance, the length-scale of the E. coli cell is about 1,000 nm (1 ?m) which is larger than, but of similar magnitude, as the diffraction limit. This explains why, prior to the development of SMLM, it was difficult to image details of the internal structures of living bacteria. Perhaps this may be the reason why bacteria are considered to be â€Å"primitive† organisms with little internal structure. With single-molecule localization, more precise structures of bacteria and other small-scale entities, e.g. individual viruses, can be resolved.In SMLM, the photochemical properties of fluorescent proteins are exploited to induce a weakly emissive or non-emissive â€Å"dark† state. From the dark state, very small populations of fluorophores are returned to an emissive state by shining a weak light pulse that activates only a fraction of the fluorophores present. These fluorophores are excited and detected by glowing until they are bleached, at which point the procedure is repeated on a new subgroup of fluorophores. In order to be identified, however, the emission profile must exhibit minimal overlap in each image. The centroid position of each identified molecule is statistically fitted, often to a Gaussian function, and with a level of precision scaling with the number of detected photons. By imaging and fitting single emitters to a sub-diffraction limited area over thousands of single images, enough data is generated to create a composite reconstruction of all identified emitters. Single-molecule localization is a broad category consisting of specific techniques, such as STORM, PALM, and GSDIM, that operate using the conceptually similar procedure outlined above. The main difference between these types is the exact fluorophore chemistry used to turn the fluorescence of individual molecules on and off. The real breakthrough in single-molecule localization occurred in 2006, when Betzig and colleagues coupled fluorescent proteins to the membrane enveloping the lysosome, the cell’s recycling station. By activating only a fraction of the proteins at a time and superimposing the individual images, Betzig ended up with a super-resolution image of the lysosome membrane. Its resolution was far better than Abbe’s diffraction limit of 0.2 ?m, a barrier that previous microscopy techniques could not bypass. Since the ground-breaking discovery, SMLM has allowed organelles and single molecules to be resolved with an order of magnitude better resolution (with a localization accuracy of about 10 nm), in multiple color channels, and in 2D as well as 3D. Single-molecule microscopy allows quantification of the number of proteins within biological assemblies and characterization of protein spatial distribution, permitting the determination of protein stoichiometry and distribution in signaling complexes. For instance, for the ?2 adrenergic receptors, SMLM was used to show that the receptors are partially organized in mini-clusters only in cardiomyocytes but not in any other cell lines, and that these oligomers are not lipid raft related but rather depend on actin cytoskeleton integrity. Most importantly, the results of this study were different from those obtained from a similar report which used near-field scanning optical microscopy (NSOM), demonstrating the better precision of SMLM over other techniques. An additional important aspect of SMLM is that it can be used with other imaging techniques to elucidate receptor complex structures. In one study by Nan et al. (2013), the powerful sensitivity of FRET imaging to detect receptor proximity was combined with the capability of SMLM to obtain direct visualization of receptor oligomers in studying RAF, a strategic protein involved in RAS signaling. By means of cluster analysis, Nan and colleagues were able to show how RAF exists between an inactive monomeric state in the cytosol and a multimeric condition at the cell membrane when activated. The results from single-molecule localization confirmed the importance of dimer and oligomer formation in RAF signaling, even though the precise biological role of these different multimeric states is yet to be determined.The better definition of biological structures in the nanometer range as a result of SMLM has had most relevance in the field of neuroscience, where the morphology of neurons composed of dendritic spines and synapses is not suitable for confocal microscopy. For example, Dani et al. (2010) used single-molecule microscopy to image presynaptic and postsynaptic scaffolding proteins in the glomeruli of the mouse olfactory bulb to show distinct punctate patterns that were not resolved by conventional fluorescence imaging. Lastly, the high resolution of SMLM has enabled a deeper understanding of chromosome organization and genome mapping. Wang et al. (2011) determined the distribution of nucleoid-associated proteins in live E. coli cells, while Baday et al. (2012) were able to label 91 out of a total of 107 reference sites on a 180 kb human BAC gene with a 100 bp resolution. DNA mapping with such resolution offers the potential to uncover genetic variance and to facilitate medical diagnosis in genetic diseases. Nonetheless, there are a few challenges that come with single-molecule microscopy, namely errors in detection efficiency and localization uncertainty. Since using fluorescent proteins as labels involves the complications associated with protein expression, errors in this step (e.g. misfolding, incomplete maturation, etc.) can lead to the production of label molecules that are not fluorescent. This can directly affect counting studies, as the number of counted molecules can be underestimated. However, it is possible to use the obtained count (after correcting for blinking artifacts) for the counting. In one study that involved identification of protein complex stoichiometry by counting photobleaching steps, Renz et al. (2012) accounted for errors in detection efficiency using a binomial model, which was found to provide accurate results. Incorporating detection efficiency into a model for the ratio between monomers and dimers can also rectify efficiency errors. In terms of localization uncertainty, each photon from the emitter molecule provides a sample of the point spread function (PSF) from the molecule. Based on these samples, single molecule localization algorithms provide an estimate for the position of the fluorescent molecule. This estimate is prone to uncertainties, especially due to limited sampling (i.e. the limited number of photons obtained from the molecule). By ensuring that the imaged molecules within a frame are spatially separated enough so that the localization algorithms can correctly identify them, however, it is possible to minimize the effect of localization uncertainty on counting measures. Despite its potential shortcomings, single-molecule localization enables high resolution imaging on the scale of nanometers, which defies Abbe’s diffraction limit of 0.2 ?m. SMLM has been used to elucidate specific cell structures, as in Betzig’s visualization of the lysosome membrane, and receptor complexes, as in the case of RAF. The technique has also been used to refute results of similar studies that used different imaging protocols, as shown when determining the specific location of ?2 adrenergic receptors. Overall, SMLM has ushered in a new era of high resolution imaging that not only allows for accurate insight into individual cell and protein structure, but also enables identification of abnormalities in cellular processes that ultimately manifest as genetic diseases. How to cite The Nobel Prize in Chemistry, Papers

Market Segmentation Conceptual and Methodological Process

Question: Discuss about the Market Segmentation for Conceptual and Methodological Process. Answer: Introduction In the mid of the 19th century, the concept of retail marketing has taken a dramatic change. The changing behaviour of customers gave birth to two of the potential phase of a retail market. Moreover, the concept of department stores and discount stores has grabbed significant attention of consumers, which provided enough space for the competition in between top companies (Zielke 2014). Department stores used to have sections for different types of products such as clothing, furniture, sporting goods and many more. Nevertheless, all necessary products assembled to a single place provided a cost effective and time saving benefit to the consumers (Davis and Dyer 2012). Moreover, they are no longer required to go to different place for different products. This assignment helps in finding out the changing face of retail management and respective customer behaviour. With the help of some potential parameters, companies are encasing on the enhanced opportunities because of a phenomenal customer response to the retail shopping. Department stores and discount stores A department store is a place, which offers wide range of consumer goods at one place. The concept has changed the shape of shopping habits in major cities in the mid of the 19th century. Department stores have different sections dedicated to a wide range of consumer goods such as clothing, home appliances and many more (Davis and Dyer 2012). Discount store is another retail concept, which offers goods at a more affordable and discounted prices (Zielke 2014). Discount stores mainly focus on the pricing of the product. However, they compromise on the quality and the service part. Aldi is a good example of such kind of a store, which has the reputation of being the world largest retailer. Moreover, it operates in more than 10,121 discount stores across the globe. Evolution of department and discount stores It all started in the mid of the 19th century, when the retail management started giving a new face to the shopping habits (Ewing 2014). Inception of department store has all changed the buying behaviour of customer by bringing more diversification in their shopping habits. Industrial revolutions have bettered the economy structure of people living in major cities. Moreover, an improved economy structure of common people acted as a catalyst in favour of the retail revolution (Mercer 2014). They sensed the gaps that need to be filled up with some new concept, which could help in enhancing the market feasibility of companies. Moreover, companies thronged to the changed concept and brought some of the best experience to the customers, which they had never imagined. Both the department stores and the discount stores are same in their product segmentation but differ in their mode of operation (Clodfelter 2012). Department stores have different sections offering wide range of consumer good s whereas discount stores offered wide range of discounted products. However, discount stores focus more on product pricing rather than the quality and the service part (Kim and Kim 2012). Aldi is a good example of such a store, which is the largest retailer in world to offer discounted products. Customer segmentation Customer segmentation refers to a process, which divides customer based on their feasible level to the pricing of the product (Wedel and Kamakura 2012). Different types of customer in Australia and Nepal represent the different face of a retail market, which solely depend on buying behaviour of customers. In Australia and Nepal, there are five types of customers in retail market such as: Potential customer: These customers come to the stores and buy the desired product irrespective of their price range (Parment 2013). In this context, Nepal has more potential customers than Australia. Moreover, this is for the reason that Nepal has less number of supermarkets than Australia. New customer: New customers are those, which gives retail market a sigh of hope of prospering in the coming future (Ferrell and Hartline 2012). However, this heavily depends on a large population of the country. Nepal has very less population than Australia as it has less number of people falling under the age of (18-30). Impulsive customer: Impulsive customer is an instant buyer of products, which happens all of sudden without any proper plan for this before (West, Ford and Ibrahim 2015). In this context, Australia has a large number of impulsive buyers because there is large number of supermarkets in the country. Customers can find various stores in various places whereas in Nepal thee are lesser number of supermarkets. Discount customer: These customers seek for a discounted product and wishes to visit to those supermarkets, which has the reputation to offer discounted products (Solomon, Russell-Bennett and Previte 2012). In this context, both Australia and Nepal have same rate of customers, who wish to buy product on a discounted price. Diversification Diversification is something, which encourages a company to bring new products in market (Oh, Sohl and Rugman 2015). Moreover, it adds another level in the business operation, which indeed is a result of added production. Diversification also helps a company during its economic downturn by bringing product variation as per the changing requirement of consumers (Goworek and McGoldrick 2015). In this regard, Aldi represent a good example, which has diversified culture in their production. The two effective issues, which are associated with the diversification, are elated issues and unrelated issues. For Aldi, the related issues are health and cosmetic products, vegetables and fresh fruits whereas unrelated issues may be electronic, stationary an household goods. The related issue with the Chaudhry Group in Nepal are house development, food and beverages. The unrelated issue for the Chaudhry Group in Nepal can be categorised as real state, automobiles, tobacco, education and many more. Moreover, the Company answered to the urgency of having brown instant noodle in Nepal, which was primarily dominated by white noodles in the 80s. Challenges and issues of retail market in Australia and Nepal Challenges and issues have always affected the business of leading companies in past (Layton 2015). In Australia also, there is a healthy competition in between different types of supermarkets and the online mode of shopping. Aldi has created a strong impact in the past by achieving the reputation of the leader company in the discount store format. However, the performance of the Company is challenged by an increasing popularity of online mode of shopping. The online mode of shopping is offering products at a cheaper rate than the discount stores. Nevertheless, Aldi is under notable pressure from diverse segments of customer in Australia (Chugh and Grandhi 2012). On a same note, Chaudhary Group is also facing certain challenges in Nepal because of a low population there. The low population in the country mean that other companies who wish to expand their business in the country might not experience a healthy market shares. Moreover, low population and challenging business access in Nepal have constructed the country as an unfavourable choice for the investors. Nonetheless, the poor transport system in Nepal has added another feather in the book of challenges in front of Chaudhry Group (Magnani, Gevorgyan and Kurz 2012). Companys Future Fresh vegetables and fruits and their environment-friendly packaging have shown Aldis utmost dedication towards the social wellbeing. Moreover, they do so to grab a competitive edge over its competitors. Aldi is strongly focussing on some key part of its production such as adding nutritional values to it with the help of extensive teams of experts who take care of the nutritional part with suitable strategies. With regular set up of different stores at different locations, Aldi has successfully hampered market shares of some leading companies such as Asda, Tesco and Morrisons during economic recession (Chugh and Grandhi 2012). On a same note, Chaudhary Group has left its massive impact on the retail market in Nepal. However, at a global level, it is facing some serious challenges such as rules regulations, cost and currency rates. Nonetheless, the Company has so far managed these challenges. CG is one of the giant companies in the Kingdom of Nepal, which has diversified inclinations across various domains. They have some of the excellent manufacturing facilities for the production of noodles, cigarettes, beverages and snacks. WAI-WAI is the market leader in Nepal in the noodle market. The Company has influenced not only Nepal but also India, Bhutan and the global countries. The effective and unique taste of the noodle has also produced worthy results in European countries such as UK, Germany Austria. Throughout the expansion of the Company during the last 137 years, the WAI-WAI has expertise in possessing not less than 40 businesses (Magnani, Gevorgyan and Kurz 2012). Cost containment and value-driven behind the changing face of retail management Cost contaminant is the business practice in order to maintain level of expense to prevent redundant expenditure in order to enhance profitability devoid of long-tem damage to the Company (Manjeshwar, Sternquist and Good 2013). Retailers mostly endeavors investments as well as operating costs with a cost-contaminant approach. Most of the retail firms use this to hold down both preliminary strategies due to intense competition from discounters (PrezCabaero, CruzRos and GonzlezCruz 2014). It is done to authorize a complicated chain or franchise operation as well as high land and construction cost and a desire to maximize the productivity. With the help of the following strategies, cost contaminant can be achieved: Regulating operating processes, size of stores, layout of stores as well as goods offerings Making the use of secondary location, self-supporting units, and sites in older stripe centers and by occupying sites deserted by others Making the use of plainer fixtures as well as lower-cost displays Making the use of reasonable construction materials that includes exposed cinder-block walls as well as solid floors. Positioning stores in smaller communities where building guidelines are less strict, cost of labor is lower and the operating cost is diminished. Purchasing renovated equipment that includes pos hardware Joining cooperative purchasing and advertising groups Encouraging manufacturers in order to finance inventories The quest to provide good value to customers acts as the driving force behind contaminant of cost (Manjeshwar, Sternquist and Good 2013). Price plays a major role in what a customer desire to acquire and from where they acquire it. However, price is no longer considered as the precise reflection of value. Changing face of retail management in coming future Concept of life cycle in retail and the wheel of retailing concept are the driving force behind the changing future of the retail market (Ryan 2016). The concept of life cycle in a retail management refers to a process through which an organization passes through. Moreover, the cycle has divided into four parts such as innovation, success, saturation and decline. Innovation is the one weapon, which a company uses to produce different expertise in the market. However, inclination of a product is also a part of the retail management, which can have various driving forces behind this such as decreasing demand for the product or changing behaviour of customers for the product (Layton 2015). On the other hand, wheel of retailing represent a strategy, which a company exhibit in their entire operation of a product in the market. They first introduce the product in market with a lower pricing to grab the attention of customers. However, once the product takes a strong foothold in the market, they increase the pricing of the product to provide them a healthy profit margin. The wheel of retailing concept is the most powerful driver behind the changing face of the retail market (Sthlberg and Maila 2012). Irrespective of the companies are following the same technique to turn consumer in their favour. Nevertheless, giving variations to the pricing of the product based on situational demand for the product produces healthy customer base (Lee and Kotler 2015). The growing awareness towards the societal values of customers has given a rethink to the companies on their products and the formats of stores. Keeping the premises environment friendly, there is an utmost requirement to maintain hygienic surroundings in the supermarkets. Nonetheless, customers are now more centric towards their health values. Scrambled merchandising When a retailer sells goods in his shop, which do not belong to the usual variety of commodities he keeps or sells it is termed as scrambled merchandizing (Zentes, Morschett and Schramm-Klein 2017). A retailer may opt for scrambled merchandizing in order to increase his top line or bottom line and for better utilization of space. Scrambled merchandizing also illustrates scattered management of product and it can leave a negative image in the mind of the customer who is not sure of what the retailer stands for (Zentes, Morschett and Schramm-Klein 2017). The observable fact is mostly observed with small and medium sized retailers whose key objective is to make more money. The modern practices among retailers in order to enlarge their product lines beyond those conventionally carried foremost competition between types of retailers. Scrambled merchandising is popular due to several causes that include the desire of the retailers to augment overall revenues, largely profitable commodities and services and because customers make more desire purchases (Sthlberg and Maila 2012). Scrambled merchandising is infectious. In other words, drugstores, florists, video-stores as well as bookstores are all affected negatively due to scrambled merchandizing. Retailers such as drugstores are forced into scrambled merchandising to complete the sales emptiness caused by supermarkets. Conclusion Although, flexibility is an important key in the changing face of the retail market but examples are very rare for such leaders. Even the giant companies like Aldi could not keep it away from certain challenges that are coming its way. The online shopping mode such as Amazon, Snapdeal, EBay and many more have all hampered the sales figure of the Company notably. To sustain the gained customer base, Aldi can go the same way by offering online product sale. They need to add online purchase mode to their existing website, which could offer wide range of products at a comparatively cheaper rate. Once it grows with the online sale system and catches significant customer base, the Company can increase respective product pricing considerably to enhance the profit margin. Low population and an unfavourable transport mean in Nepal are causing serious threat to the Chaudhry Group. Big companies are not daring to expose their expertise in this counterpart. Nonetheless, due to low population companies have less scope for their drive in market shares. CG can go for a merger concept as this can expose their expertise in front of international customers. Moreover, this can open ample of options to the Chaudhry Group in international market with diverse range of products. The concept of retail market is continually changing, which is driven by certain forces such as cost containment, value-driven and scrambled merchandising. However, changing customer behaviour is acting as the most influential factor for a changing face of retail market in the coming future. The diverse culture of buyers has opened up several dimensions for a retail market expansion such as online sales of goods through various platforms. Moreover, Amazon, Snapdeal, EBay and many more have changed the buying perceptions of customers in a big deal. A considerable growth is there in the online buyers list, which is providing ample of space to the existing giants in retail market to look for a significant change in their operations mode. Nonetheless, Aldi in Australia and Chaudhry Group in Nepal both need to improve their operation mode to meet with the enhanced demand of customer buying habit. References: Chugh, R. and Grandhi, S., 2012. E-Tailing: A Snapshot of Australias Top 25 Retailers.Strategic and Pragmatic E-Business: Implications for Future Business Practices: Implications for Future Business Practices, p.297. Clodfelter, R., 2012.Retail buying: from basics to fashion. AC Black. Davis, L.Y. and Dyer, B., 2012. Consumers' value perceptions across retail outlets: shopping at mass merchandisers and department stores.The International Review of Retail, Distribution and Consumer Research,22(2), pp.115-142. Ewing, E., 2014.History of 20th century fashion. Pavilion Books. Ferrell, O.C. and Hartline, M., 2012.Marketing strategy, text and cases. Nelson Education. Goworek, H. and McGoldrick, P., 2015.Retail Marketing Management: Principles and Practice. Pearson Higher Ed. Kim, J.E. and Kim, J., 2012. Human factors in retail environments: a review.International journal of retail distribution management,40(11), pp.818-841. Layton, R.A., 2015. Formation, growth, and adaptive change in marketing systems.Journal of Macromarketing,35(3), pp.302-319. Lee, N.R. and Kotler, P., 2015.Social marketing: Changing behaviors for good. Sage Publications. Magnani, R., Gevorgyan, A. and Kurz, K., 2012. Market analysis of complementary foods in Nepal.Global Nutrition Collaborative Research Support Program (CRSP) Research Briefing Paper, (12). Manjeshwar, S., Sternquist, B. and Good, L.K., 2013. Decision making of retail buyers: perspectives from China and India.Qualitative Market Research: An International Journal,16(1), pp.38-52. Mercer, H., 2014. Retailersupplier relationships before and after the Resale Prices Act, 1964: A turning point in British economic history?.Enterprise and Society,15(01), pp.132-165. Oh, C.H., Sohl, T. and Rugman, A.M., 2015. Regional and product diversification and the performance of retail multinationals.Journal of International Management,21(3), pp.220-234. Parment, A., 2013. Generation Y vs. Baby Boomers: Shopping behavior, buyer involvement and implications for retailing.Journal of retailing and consumer services,20(2), pp.189-199. PrezCabaero, C., CruzRos, S. and GonzlezCruz, T., 2014. The contribution of dynamic marketing capabilities to service innovation and performance.International Journal of Business Environment,7(1), pp.61-78. Ryan, D., 2016.Understanding digital marketing: marketing strategies for engaging the digital generation. Kogan Page Publishers. Solomon, M., Russell-Bennett, R. and Previte, J., 2012.Consumer behaviour. Pearson Higher Education AU. Sthlberg, M. and Maila, V., 2012.Shopper marketing: How to increase purchase decisions at the point of sale. Kogan Page Publishers. Wedel, M. and Kamakura, W.A., 2012.Market segmentation: Conceptual and methodological foundations(Vol. 8). Springer Science Business Media. West, D., Ford, J. and Ibrahim, E., 2015.Strategic marketing: creating competitive advantage. Oxford University Press. Zentes, J., Morschett, D. and Schramm-Klein, H., 2017. Store-based RetailingGeneral Merchandise. InStrategic Retail Management(pp. 47-70). Springer Fachmedien Wiesbaden. Zielke, S., 2014. Shopping in discount stores: The role of price-related attributions, emotions and value perception.Journal of Retailing and Consumer Services,21(3), pp.327-338.

Problem With Educational System

Question: Discuss about the Problem With Educational System. Answer: Introduction The system of public schooling within any of the economic system which is not a private one can be referred as educational system (Moore and Kearsley, 2011). Schools are to be considered as the smallest forms of this system and economies are to be the largest one. The below presented essay has been focused and analysed the problems with the educational system. Problems with the Educational System Academics is to be considered as the core term or the centre of attention around which the whole educational system revolves and yet the system fails to fulfill the needs of the kids. The best part to be considered of the educational system is that it is over-rated and the worst is that it has destroyed a number of kids. It is very of much significance for the students to learn how to read and write and also other valuable skills. But instead of doing this the students should learn how to learn (Brown, Harris and Russell, 2010). Now-a-days the students have just concentrated themselves on filling their journals, worksheets and copying down lectures notes which are easily available on google with one click. Most of the time the students find the lectures boring, irrelevant and are not connected to their lives and the content taught in these lectures is memorized for the exams and which some-times can be easily forgotten, there is a big question and risk of failure for the students. Being academic is not enough as it doesn't tell much about yourself, it will just tell you about how good the students are in their school life. But it does not indicate how successful will be the married life, raising the kids or how wealthy will you be in future and what position will you be having in your society. And these are all the topics which are considered as important in comparison to how good you were at your school time. School should be made a place where students can discover what they actually love and should be able to ask questions which are relevant and can easily pursue answers of them (Wright, 2013). The students should be made so capable of discovering what they are passionate about, in doing what they feel happiness, their soul feels relaxed. A human being is born curious. A new born baby explores the environment and do it natrually without being told. Till the age of three they constantly have the question of Why and by the time they reach grade 10 they have all their answers but left with zero level of curiosity (Hargreaves et al, 2014).The schools should be replaced from just learning of 10 years to a teaching which lasts lifelong. The era has began to move so fast that by the time a student graduates from university everything he or she learned may already be headed towards obsocelence. Conclusion From the above carried out analysis it can be concluded that the educational system is facing a number of problems and it has just become a schooling session where how to read and write is taught and the whole concept of educational system just revolves around the word academics. And there are a numerous problems associated with this system. References Moore, M.G. and Kearsley, G., 2011. Distance education: A systems view of online learning. Cengage Learning. Brown, V.A., Harris, J.A. and Russell, J.Y., 2010. Tackling wicked problems through the transdisciplinary imagination. Earthscan. Hargreaves, A., Lieberman, A., Fullan, M. and Hopkins, D.W. eds., 2014. International handbook of educational change: Part two (Vol. 5). Springer. Wright, S, 2013, Academic Teaching Doesn't Prepare Students for life, Retrieved on 17th Febraury 2017. https://plpnetwork.com/2013/11/07/obsession-academic-teaching-preparing-kids-life/.